CHU (N6) medium including vitamins - Duchefa
Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.
| Micro Elements | mg/l | µM |
|---|---|---|
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 1.60 | 25.88 |
| KI | 0.80 | 4.81 |
| MnSO4.H2O | 3.33 | 19.70 |
| ZnSO4.7H2O | 1.50 | 5.22 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 125.33 | 1.13 |
| KH2PO4 | 400.00 | 2.94 |
| KNO3 | 2830.00 | 27.99 |
| MgSO4 | 90.27 | 0.75 |
| (NH4)2SO4 | 463.00 | 3.50 |
| Vitamins | mg/l | µM |
|---|---|---|
| Glycine | 2.00 | 26.64 |
| Thiamine HCl | 1.00 | 2.96 |
| Pyridoxine HCl | 0.50 | 2.43 |
| Nicotinic acid | 0.50 | 4.06 |
Total concentration Micro and Macro elements including -vitamins: 3956.53 mg/l
Chu C.C, The N6 medium and its application to anther culture of -cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen -sources. Scienta Sinic., 18, 659 (1975).