Eriksson (ER) medium - Duchefa
SKU
BIO-E0207
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Product description
Eriksson medium was developed for cell suspension cultures of Haplopappus gracilis.
An increase in the growth of cell suspensions, especially with small inocula, was achieved by a 10% reduction of the concentration MS microelements, except for Fe and Zn. Equim-olar replacement of ZnSO4.-7H2O by ZnNa2EDTA improved cell growth as well. MnEDTA and CoEDTA did not improve the growth of the cell culture. A reduction of Murashige and Skoog NH4NO3 concentrati-on of 1650 to 1200 mg/l and an increase in phosphate to 2.5 mM also stimulated cell growth.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.0025 | 0.01 |
| CuSO4.5H2O | 0.0025 | 0.01 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 0.63 | 10.19 |
| MnSO4.H2O | 1.69 | 10.00 |
| Na2MoO4.2H2O | 0.025 | 0.10 |
| ZnSO4.7H2O | 9.15 | 31.80 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | 2.99 |
| KH2PO4 | 340.00 | 2.50 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 1200.00 | 14.99 |
Eriksson T., Physiol. Plant, 18, 976 (1965).