Gresshoff & Doy medium - Duchefa
The medium defined by Gresshoff and Doy is developed for growth of haploid callus and plants of Arabidopsis thaliana cultured from the diploid anthers. The anthers were removed during the late prophase of meiosis, selecting a genotype favouring callus formation from dividing sporocytes on a high auxin - low kinetin concentration in a fully defined medium. Further differentiati-on was induced by transfer to a low auxin - high kinetin medium with a light-dark cycle. Haploid callus cultures of tomato, barley and Vitis vinifera have been cultured as well using this method.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 0.30 | 4.85 |
| KI | 0.80 | 4.82 |
| MnSO4.H2O | 1.00 | 5.92 |
| Na2MoO4.2H2O | 0.025 | 0.10 |
| ZnSO4.7H2O | 0.30 | 1.04 |
| Macro Elements | mg/l | mM |
|---|---|---|
| Ca(NO3)2.2H2O | 208.81 | 1.04 |
| KCl | 65.00 | 0.87 |
| KH2PO4 | 300.00 | 2.20 |
| KNO3 | 1000.00 | 9.89 |
| MgSO4 | 17.09 | 0.14 |
| NH4NO3 | 1000.00 | 12.49 |
Gresshoff P.M. et al., Haploid Arabidopsis thaliana callus and plants from anther culture. Aust. J. Biol. Sci, 25, 259 (1972). Gresshoff P.M. et al. , Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera, Z. Pflanzenphysiol. 73, 132-141, (1974).