NLN medium - Duchefa
The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg/l. To prepare the proper NLN medium formulation 500 mg/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg/l. To prepare the proper NLN medium formulation 500 mg/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 10.00 | 161.73 |
| MnSO4.H2O | 18.95 | 111.94 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 10.00 | 34.78 |
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 10.00 | 161.73 |
| MnSO4.H2O | 18.95 | 111.94 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 10.00 | 34.78 |
| Macro Elements | mg/l | mM |
|---|---|---|
| Ca(NO3)2.4H2O | Ommitted (500 mg/l) | |
| KH2PO4 | 125.00 | 0.92 |
| KNO3 | 125.00 | 1.24 |
| MgSO4 | 61.00 | 0.51 |
| Ca(NO3)2.4H2O | Ommitted (500 mg/l) | |
| KH2PO4 | 125.00 | 0.92 |
| KNO3 | 125.00 | 1.24 |
| MgSO4 | 61.00 | 0.51 |
Total concentration Micro and Macro elements: 386.95 mg/l
Total concentration Micro and Macro elements: 386.95 mg/l
Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982
Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water