Schenk & Hildebrandt medium - Duchefa

SKU
BIO-S0225

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Product description

Schenk en Hildebrandt medium has been developed for growth of both monocotyle and dicotyle cell suspensions. A high level of auxin-type growth regulators, 2,4-D (0.5 mg/l) and 4-CPA (2.0 mg/l), g-enerally favoured monocoty-ledonous cell cul-tu-r-es, while low levels of cytokin, kinetin (0.1 mg/l), were essential for most dicotyledonous cell cultures.

Micro Elements mg/l µM
CoCl2.6H2O 0.10
CuSO4.5H2O 0.20
FeNaEDTA 19.80
H3BO3 5.00
KI 1.00
MnSO4.H2O 10.00
Na2MoO4.2H2O 0.10
ZnSO4.7H2O 1.00
Macro Elements mg/l mM
CaCl2 151.00
KNO3 2500.00
MgSO4 195.05
(NH4)H2PO4 300.00
Vitamins mg/l µM
myo-Inositol 1000.0
Nicotinic acid 5.0
Pyridoxine HCl 0.5
Thiamine HCl 5.0

Total concentration Micro and Macro elements including vitamins: 4193.75mg/l

Schenk R.U. and Hildebrandt A.C., Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50, 199 (1972).

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water